Table 3. Effect of PEF on the proteolysis of different muscles

Meat materials PEF condition Target protein Findings References
Semitendinosus (beef) 30 μs, 50 Hz, 0.5 to 2.0 kV/cm, 100 pulses μ-Calpain, troponin-T μ-Calpain was not reduced regardless of PEF intensity, however, troponin-T was proportionally reduced by increases of PEF intensity. Jeong et al. (2023)
Myofibrillar protein (MP) extraction of longissimus lumborum (pork) 6 μs, 500 Hz, 2.5 to 7.5 kV/cm, 15,000 to 45,000 pulses MP Microstructure of MP was deformed, and the hydrophobicity of MP surface was enhanced by PEF treatment. Wang et al. (2022)
MP extraction from the pectoralis (chicken) 600 to 1,000 Hz, 8 to 28 kV/cm, pulse width and number of pulses are not described MP The solubility and hydrophobicity of MP were increased, and the dynamic rheological properties were enhanced by PEF treatment. Dong et al. (2020)
Longissimus lumborum (deer) 20 μs, 50 Hz, 2.5 to 10 kV, treatment time or number of pulses are not described Myofibril The myofibril was fragmented and ruptured along the z-line by PEF treatment. Mungure et al. (2020)
Longissimus thoracis (beef) 20 μs, 50 Hz, 1.0 and 1.25 kV/cm, 500 and 2,000 pulses Myosin, actin, collagen, myofibril Thermal profiles of the myosin, actin, and collagen were not affected by PEF treatment.The myofibrillar structure was ruptured by PEF treatment. Chian et al. (2019)
Longissimus dorsi (deer) 20 μs, 50 and 90 Hz, 0.2 and 0.5 kV/cm, 1,500 and 2,700 pulses Calpain, desmin, troponin-T The calpain activity was slightly increased by PEF treatment.The degradation of desmin and troponin-T was observed in PEF treated sample. Bhat et al. (2019c)
Longissimus dorsi (deer) 20 μs, 50 and 90 Hz, 0.3125 and 1.25 kV/cm, 1,500 and 2,700 pulses Meat protein Free amino acids The digestibility and solubility of meat protein were increased by PEF treatment.The concentration of free amino acids was higher in the PEF treated sample. Bhat et al. (2019b)
Longissimus lumborum and semimembranosus (beef) 20 to 90 Hz, 0.625 and 1.25 kV/cm, pulse width and number of pulses are not provided Troponin-T Desmin The degradation of desmin and troponin-T was increased by PEF treatment. Suwandy et al. (2015a)
Longissimus lumborum and semimembranosus (beef) 20 to 90 Hz, 5 and 10 kV/cm, pulse width and number of pulses are not provided Troponin-T Desmin The degradation of desmin and troponin-T was increased by PEF treatment. Suwandy et al. (2015b)
Semitendinosus (beef) 20 μs, 5 to 200 Hz, 1.1 to 2.8 kV/cm, 152 to 300 pulses Myofibril fragment The particle size of filtered myofibril fragment was decreased by PEF treatment. O’Dowd et al. (2013)
PEF, pulsed electric field.