| Instrument | Agilent 7890A GC | Agilent 7890A GC | Agilent 7890A GC |
| Detector | Flame ionization detector (FID) | Flame ionization detector (FID) | Flame ionization detector (FID) |
| Column | SPTM-2560 Capillary GC column (Supelco 24056) (100 m × 0.25 mm × 0.20 μm) | HT-5 (Aluminum clad) Capillary GC column (Supelco 25003) (25 m × 0.32 mm × 0.10 μm) | HP-5 5% Phenyl methyl siloxane (Agilent 19091J-413) (30 m × 0.32 mm × 0.25 μm) |
| Inlet temperature | 250℃ | 380℃ | 280℃ |
| Injection Volume | 1 μL | 2 μL | 1 μL |
| Inlet split ratio | 50 : 1 | 50 : 1 | 5 : 1 |
| Carrier gas | N2 (Nitrogen) | N2 (Nitrogen) | N2 (Nitrogen) |
| Oven condition | 180℃ for 40 min then 3℃/min to 230℃ for 10 min | 340℃ for 1 min then 5℃/min to 370℃ for 13 min | 250℃ for 25 min then 10℃/min to 290℃ for 9min |
| Detector Temp. | 250℃ | 380℃ | 290℃ |
| Flow rate | 1.0 mL/min. | 1.8 mL/min | 1.0 mL/min |
| Run time | 67 min | 20 min | 38 min |