Table 1. Conditions for enrichment, isolation, and confirmation of 9 kinds of foodborne pathogenic microorganisms
Microorganisms | Enrichment | Isolation | Confirmation |
|
Medium | Condition | Medium | Condition |
B. cereus | - | - | Mannitol egg yolk | 30°C, 24 h | VITEC 2–compact |
polymyxin agar |
C. perfringens | Cooked meat agar | 37°C, 24 h, | Perfringens agar base | 37°C, 24 h | VITEC 2–compact |
anaerobic |
C. jejuni | Bolton broth | 37°C, 5 h, | | | VITEC 2–compact |
microaerophilic | Modified Campy blood | 42°C, 48 h, |
42°C, 48 h, | free agar base | microaerophilic |
microaerophilic | | |
E. coli O157:H7 | mTSB* | 37°C, 24 h | TC-SMAC*, BCIG* | 37°C, 24 h | VITEC 2–compact |
L. monocytogenes | UVM-modified Listeria | 30°C, 24 h | Oxford agar | 30°C, 24 h | API Listeria |
selective agar base |
Salmonella spp. | Peptone water, | 37°C, 24 h | MacConkey | 37°C, 24 h | VITEC 2–compact |
Rappaport-Vassiliadis | 42°C, 24 h |
S. aureus | TSB* | 37°C, 24 h | Baird-Parker agar | 37°C, 24 h | VITEC 2–compact |
Y. enterocolitica | PSBB* | 10°C, 10 d | MacConkey, CIN* | 30°C, 24 h | VITEC 2–compact |
V. parahaemolyticus | Alkaline peptone water | 37°C, 24 h | TCBS* | 37°C, 24 h | VITEC 2–compact |