| Gene | Sequence of PCR Primer |
|---|---|
| ZO-1 | Forward: 5'-GCCTCTGCAGTTAAGCAT-3' |
| Reverse: 5'-AAGAGCTGGCTGTTTTAA-3' | |
| Occludin | Forward: 5'-CTGTCTATGCTCGTCATCG-3' |
| Reverse: 5'-CATTCCCGATCTAATGACGC-3' | |
| Claudin | Forward: 5'-ATGGTAGCCACTTGCCTTC-3' |
| Reverse: 5'-TTAGACATGGGCACCTTGG-3' | |
| TNF-α | Forward: 5'-AAATGGGCTCCCTCTCATCAGTTC-3' |
| Reverse: 5'-TCTGCTTGGTGGTTTGCTACGAC-3' | |
| β-actin | Forward: 5'-AGAGCTATGAGCTGCCTGAC-3' |
| Reverse: 5'-CTGCATCCTGTCAGCGTACG-3' |