Table 2 Effect of different packaging methods on microbiological characteristics during storage at 4°C

Parameter/time (day)	Packaging
Parameter/time (day)	WP	MAP	VP1	VP2	VP3	VSP
TVC
0	<2.00^E	<2.00^C	<2.00^E	<2.00^D	<2.00^D	<2.00^E
7	4.55±0.10^Dc	5.76±0.08^Aa	5.12±0.08^Db	2.74±0.04^Ce	2.30±0.30^Ce	3.30±0.12^Dd
14	6.59±0.12^Ca	4.87±0.02^Bd	5.50±0.05^Cc	5.89±0.19^Ab	6.06±0.06^Bb	4.23±0.03^Ce
21	7.68±0.07^Aa	5.10±0.04^Be	6.74±0.04^Bb	5.93±0.03^Ad	6.60±0.01^Ac	5.82±0.00^Ad
28	6.93±0.08^Bb	5.98±0.13^Ac	7.26±0.01^Aa	5.49±0.07^Bd	6.67±0.07^Ab	4.96±0.12^Be
PSY
0	<2.00^D	<2.00^D	<2.00^E	<2.00^E	<2.00^D	<2.00^E
7	4.43±0.02^Cc	5.68±0.01^Ba	5.21±0.00^Db	2.74±0.04^Df	3.35±0.12^Ce	3.59±0.02^Dd
14	6.18±0.39^Bb	5.02±0.02^Cc	5.73±0.02^Cb	6.13±0.02^Ab	6.81±0.04^Ba	4.75±0.10^Cc
21	7.72±0.08^Aa	5.01±0.09^Cd	6.74±0.04^Bb	5.92±0.05^Bc	6.88±0.08^Bb	5.76±0.04^Ac
28	7.85±0.02^Aa	6.92±0.04^Ac	7.36±0.04^Ab	5.45±0.03^Cd	7.78±0.09^Aa	5.54±0.05^Bd
LAB
0	<2.00^E	<2.00^D	<2.00^D	<2.00^C	<2.00^E	<2.00^E
7	2.80±0.10^Da	3.01±0.03^Ca	3.03±0.10^Ca	2.24±0.24^Bb	2.00±0.00^Db	2.98±0.20^Da
14	4.65±0.07^Cc	4.67±0.03^Bc	5.56±0.02^Bb	5.89±0.19^Aa	6.06±0.06^Ba	4.23±0.03^Cd
21	6.93±0.04^Aa	5.12±0.16^Acd	6.31±0.71^ABab	5.92±0.01^Aabc	4.62±0.01^Cd	5.70±0.04^Abc
28	6.20±0.03^Bb	5.47±0.15^Ac	7.24±0.03^Aa	5.54±0.13^Ac	7.52±0.07^Aa	4.78±0.18^Bd
COL
0	<2.00^D	<2.00^D	<2.00^B	<2.00^B	<2.00	<2.00
7	<2.00^D	<2.00^D	<2.00^B	<2.00^B	<2.00	<2.00
14	3.64±0.07^Ca	2.90±0.05^Cb	<2.00^Bc	<2.00^Bc	<2.00^c	<2.00^c
21	4.61±0.14^Aa	3.54±0.06^Ab	<2.00^Bc	<2.00^Bc	<2.00^c	<2.00^c
28	4.23±0.01^Ba	3.33±0.10^Bb	3.42±0.42^Ab	2.65±0.05^Ac	<2.00^d	<2.00^d
ANB
0	<2.00^D	<2.00^D	<2.00^E	<2.00^E	<2.00^D	<2.00^E
7	4.61±0.03^Ca	3.34±0.14^Cc	3.77±0.00^Dbc	2.94±0.24^Dc	2.39±0.09^Cd	3.37±0.03^Dbc
14	6.69±0.00^Ba	4.76±0.05^Be	5.77±0.04^Cd	6.47±0.02^Ab	6.10±0.02^Bc	4.46±0.00^Cf
21	7.71±0.04^Aa	5.03±0.08^Be	6.75±0.03^Bb	5.87±0.06^Bd	6.54±0.08^Ac	5.70±0.04^Ad
28	6.74±0.06^Bb	6.13±0.17^Ac	7.19±0.02^Aa	5.36±0.00^Cd	6.27±0.16^ABc	4.98±0.02^Be

All values are means±standard deviation of three replicates.
^A-E and ^a-e in the same column and row are significantly different (p<0.05), respectively.
WP, wrapped packaging; MAP, modified atmosphere packaging; VP1, VP2, and VP3, vacuum packaging with different vacuum films; VSP, vacuum skin packaging; TVC, total viable count; PSY, psychrotrophic bacteria; LAB, lactic acid bacteria; COL, coliform; ANB, anaerobic bacteria.