Table 1. Accuracy, precision, recovery, and detection and quantitative limits of nucleotides using high-performance liquid chromatography with diode array detector (HPLC-DAD)

Nucleotides	Spiked amount (mg/kg)	Intra-day		Inter-day		LOD (mg/L)	LOQ (mg/L)	Coefficient correlation (r²)	Recovery²⁾(%)
Nucleotides	Spiked amount (mg/kg)	Accuracy¹⁾(%)	Precision (CV, %)	Accuracy (%)	Precision (CV, %)	LOD (mg/L)	LOQ (mg/L)	Coefficient correlation (r²)	Recovery²⁾(%)
CMP	2	101.06±1.43	1.51	102.72±1.71	3.33	0.09	0.31	0.9999	101.06±1.43
UMP	2	84.90±2.27	3.27	84.69±1.06	6.82	0.11	0.37	0.9999	84.90±2.27
AMP	2	96.18±3.59	3.71	98.01±1.84	2.33	0.12	0.38	0.9999	96.18±3.59
GMP	2	96.62±3.64	1.56	99.26±2.32	2.79	0.11	0.39	0.9999	96.62±3.64
IMP	2	102.28±1.52	5.21	99.33±2.89	4.20	0.14	0.45	0.9999	102.28±1.52

Accuracy=Measured concentration/Spiked concentration (Mean).
Mean±SD (n=3).
HPLC-DAD, high-performance liquid chromatography with diode array detector; CV, SD of measured concentration/means of measured concentration; LOD, limit of detection, signal-to-noise (S/N) ratio=3; LOQ, limit of quantitation, S/N ratio=10; CMP, cytidine 5′-monophosphate; UMP, uridine 5′-monophosphate; AMP, adenosine 5′-monophosphate; GMP, guanosine 5′-monophosphate; IMP, inosine 5′-monophosphate.