Table 1. Antimicrobial activity of the cell free supernatant (CFS) of the Leuconostoc isolates against Listeria monocytogenes MTCC 657 in spinach stored at 4°C for a period of 5 days represented as average Log10 CFU/mL of rinse of the different groups at different time points
Groups | Log10 CFU/mL of rinse1) | % of reduction over control2) |
0 d | 1 d | 2 d | 5 d | 0 d (%) | 1 d (%) | 2 d (%) | 5 d (%) |
Control | 5.81± 0.08a,x | 5.91±0.04a,x | 5.89±0.08a,x | 5.85±0.09a,x | N/A | N/A | N/A | N/A |
CM17 CFS | 5.7± 0.24a,x | 5.38±0.16c,xy | 5.42±0.04b,y | 5.35±0.12bc,y | 22.3 | 71.0 | 65.8 | 68.7 |
CM19 CFS | 5.73±0.07a,x | 5.75±0.13b,x | 5.42±0.08b,y | 5.41±0.03b,y | 18.0 | 30.8 | 66.3 | 64.2 |
PM30 CFS | 5.79±0.06a,x | 5.35±0.10c,y | 5.09±0.07bc,yz | 5.01±0.014cd,z | 6.2 | 72.9 | 84.1 | 85.8 |
PM32 CFS | 5.76±0.11a,x | 5.46±0.10c,y | 5.39±0.07bc,y | 5.28±0.06bcd,y | 11.3 | 65.0 | 68.4 | 73.2 |
PM36 CFS | 5.78±0.09a,x | 5.34±0.29c,y | 5.24±0.24c,y | 4.86±0.07d,y | 7.2 | 73.3 | 77.8 | 90.0 |
n=3, p<0.05.
Cells with different alphabets represent statistical difference.
represents statistical difference between the groups at a specific testing period whereas
represents statistical difference within a group over time.
The % of reduction represented for the antimicrobial treatment groups indicate % reduction over the control at a specific testing time point.
MTCC, microbial type culture collection and gene bank.