Table 2. Antimicrobial activity of the cell free supernatant (CFS) of the Leuconostoc isolates against Escherichia coli MTTC 443 in spinach stored at 4°C for a period of 5 days represented as average Log10 CFU/mL of rinse of the different groups at different time points
Groups | Log10 CFU/mL of rinse1) | % of reduction over control2) |
0 d | 1 d | 2 d | 5 d | 0 d (%) | 1 d (%) | 2 d (%) | 5 d (%) |
Control | 7.29±0.10a,x | 7.32±0.15a,x | 7.41±0.13a,x | 7.51±0.19a,x | N/A | N/A | N/A | N/A |
CM17 CFS | 7.16±0.05a,x | 6.79±0.14bc,y | 6.61±0.15b,y | 6.52±0.16b,y | 26.4 | 70.5 | 84.0 | 89.9 |
CM19 CFS | 7.03±0.06a,x | 6.90±0.08b,y | 6.79±0.07b,y | 6.82±0.04b,y | 44.9 | 61.7 | 76.2 | 79.7 |
PM30 CFS | 7.18±0.34a,x | 6.67±0.17bc,y | 6.45±0.62b,y | 6.19±0.15b,y | 23.5 | 77.7 | 89.0 | 95.2 |
PM32 CFS | 6.97±0.06a,x | 6.79±0.07bc,y | 6.82±0.05b,y | 6.56±0.09b,y | 52.9 | 70.3 | 74.4 | 88.9 |
PM36 CFS | 7.12±0.32a,x | 6.38±0.01c,y | 6.04±0.07b,y | 5.94±0.04b,y | 33.5 | 88.4 | 95.7 | 97.4 |
n=3, p<0.05.
Cells with different alphabets represent statistical difference.
represents statistical difference between the groups at a specific testing period whereas
represents statistical difference within a group over time.
The % of reduction represented for the antimicrobial treatment group indicates % reduction over the control at a specific testing time point.