Table 4. Amino acid composition (%) of non-hydrolysed elastin and its fractions obtained from ultrafiltration and gel filtration

AA	UEB (%)	EBH III (%)	EB-II (%)
Asp	7.35±0.04^b	9.86±0.01^a	6.99±0.01^c
Ser	3.01±0.12^a	2.85±0.06^b	2.78±0.01^b
Gly	18.36±0.44^c	20.64±0.01^a	19.63±0.01^b
Glu	11.28±0.2^b	9.86±0.01^c	12.31±0.01^a
His	1.6±0.03^b	1.58±0.01^b	1.86±0.01^a
Arg	7.89±0.27^a	6.67±0.01^c	7.07±0.01^b
Thr	1.56±0.04^a	1.13±0.01^b	1.35±0.01^b
Ala	8.13±0.08^c	8.41±0.01^b	8.29±0.01^b
Pro	10.84±0.12^a	11.38±0.07^b	10.52±0.01^b
Tyr	1.65±0.03^b	1.7±0.01^a	4.92±0.01^a
Val	2.99±0.07^a	2.57±0.01^a	2.4±0.01^a
Met	1.72±0.11^a	1.73±0.01^a	0.89±0.01^b
Lys	4.08±0.27^a	4.3±0.01^a	4.15±0.01^a
Ile	1.72±0.11^c	1.98±0.01^a	1.82±0.01^b
Leu	4.24±0.04^a	3.46±0.01^b	3.35±0.05^b
Phe	0.24±0.16^c	1.51±0.01^b	2.7±0.01^a
Cys	0.14±0.1^a	0.11±0.01^a	0.2±0.01^a
Trp	0.14±0.1^a	0.11±0.01^a	0.2±0.01^a
Hyp	10.4±0.27^a	7.87±0.01^b	5.68±0.01^c

All values are mean±SD of three replicates (n=3).
Hydrophobic amino acids (Gly, Ala, Val, Leu, Ile, Pro, Phe, Cys, Trp, and Met).
Mean in the same row without a common superscript letter differ significantly (p<0.05).
AA, amino acid; UEB, non-hydrolysed elastin; EBH, elastin-based hydrolysate.