Table 5. Antimicrobial effects of porcine blood protein hydrolysates hydrolyzed by six proteases (2 h) (cm)
Pathogenic bacteria | Proteins | Hydrolysates | 50 μL (20 mg/mL) | 200 μL (80 mg/mL) | 400 μL (160 mg/mL) |
Enzymes | Diameter | Diameter | Diameter |
B. cereus | Albumin | Alcalase | 1.45±0.07 | 2.05±0.05 | 2.35±0.21 |
Neutrase | 1.50±0.00 | 1.95±0.07 | 2.10±0.14 |
Flavourzyme | 1.35±0.06 | 2.30±0.14 | 2.35±0.07 |
Protamex | 1.25±0.03 | 2.15±0.21 | 2.30±0.14 |
Trypsin | 1.30±0.14 | 2.35±0.05 | 2.55±0.06 |
Papain | - | - | - |
Globulin | Alcalase | - | 1.30±0.10b | 1.53±0.06b |
Neutrase | - | 1.21±0.08b | 1.68±0.08a |
Flavourzyme | 0.09±0.00b | 1.58±0.08a | 1.72±0.03a |
Protamex | 0.85±0.07b | 1.58±0.08a | 1.75±0.05a |
Trypsin | 1.05±0.07a | 1.58±0.08a | 1.800±.10a |
Papain | - | - | - |
Plasma | Alcalase | - | 1.43±0.06 | 1.75±0.05 |
Neutrase | 0.90±0.10 | 1.47±0.06 | 1.73±0.06 |
Flavourzyme | - | 1.47±0.06 | 1.67±0.06 |
Protamex | 0.87±0.06 | 1.53±0.06 | 1.77±0.06 |
Trypsin | - | 1.57±0.06 | 1.73±0.06 |
Papain | - | - | - |
Control | Antibiotics (μL) | | 5 | 10 | 50 |
Bacillus cereus | Ampicillin (50 mg/mL) | | - | 1.50±0.05 | 1.84±0.07 |
Staphylococcus aureus | Ampicillin (50 mg/mL) | | 2.540.09 | 2.81±0.04 | 2.96±0.07 |
Salmonella Typhimurium | Ampicillin (50 mg/mL) | | 1.14±0.05 | 2.60±0.05 | 2.84±0.10 |
Escherichia coli | Ampicillin (50 mg/mL) | | 2.06±0.08 | 2.21±0.09 | 2.55±0.06 |
Shigella flexneri | Ampicillin (50 mg/mL) | | 1.80±0.07 | 2.20±0.06 | 2.72±0.09 |
Means with different superscription within the same column differ (p<0.05).