Table 1. Primer sequences used in quantitative real-time PCR

Gene	Description	Forward primer (5’ → 3’)	Reverse primer (5’ → 3’)
gtfA	Sucrose phosphorylase	AGGAAGTGAAGCGGCCAGT	TCAATACGGCCATCCAAATCA
gtfB	Glucosyltransferase B	AGCAATGCAGCCAATCTACAAAT	ACGAACTTTGCCGTTATTGTCA
gtfD	Glucosyltransferase-I	ACAGCAGACAGCAGCCAAGA	ACTGGGTTTGCTGCGTTTG
ftf	Fructosyltransferase	AAATATGAAGGCGGCTACAACG	CTTCACCAGTCTTAGCATCCTGAA
relA	Guanosine tetra (penta)-phosphatesynthetase	ACAAAAAGGGTATCGTCCGTACAT	AATCACGCTTGGTATTGCTAATTG
vicR	Histidine kinase two-component regulatory system	TGACACGATTACAGCCTTTGATG	CGTCTAGTTCTGGTAACATTAAGTCCAATA
brpA	Biofilm-regulation protein	GGAGGAGCTGCATCAGGATTC	AACTCCAGCACATCCAGCAAG
comDE	Competence-stimulating system	ACAATTCCTTGAGTTCCATCCAAG	TGGTCTGCTGCCTGTTGC
16S rRNA	16S ribosomal RNA, normalizing internal standard	CCTACGGGAGGCAGCAGTAG	CAACAGAGCTTTACGATCCGAAA