Table 2. Comparison of the sensitivity of seven commercial master mixes for detecting porcine DNA by real-time PCR

Manufacturer	Platform	CT values for different concentrations of porcine DNA (ng/μL)
		5×101	5×100	5×10–1	5×10–2	5×10–3	5×10–4
Applied Biosystems	StepOnePlus	17.77±0.53	20.60±0.47	23.97±0.46	28.44±0.93	31.00±0.08	34.79±0.33
	CFX Connect	17.38±0.23	20.01±0.18	24.65±0.04	27.92±0.32	32.15±0.04	ND
CancerROP	StepOnePlus	18.54±0.09	20.56±0.12	25.44±0.47	29.18±0.48	32.09±0.72	ND
	CFX Connect	15.72±0.44	18.23±0.64	22.54±0.52	26.52±0.02	29.91±0.17	33.41±0.52
Invitrogen	StepOnePlus	17.01±0.16	19.79±0.34	23.71±0.21	26.91±0.22	30.86±0.30	33.78±0.21
	CFX Connect	17.60±0.34	21.05±0.02	24.57±0.13	28.45±0.13	31.40±0.04	34.75±0.13
Kogene Biotech	StepOnePlus	15.35±0.37	18.59±0.35	21.96±0.64	25.13±0.15	28.92±0.05	32.01±0.08
	CFX Connect	17.40±0.45	20.55±0.42	23.97±0.41	27.20±0.03	30.75±0.12	33.81±0.43
New England Biolabs	StepOnePlus	17.27±0.04	19.82±0.24	24.39±0.09	27.85±0.05	31.16±0.19	34.38±1.03
	CFX Connect	17.61±0.11	19.11±0.69	22.38±0.34	25.89±0.48	29.65±0.54	32.56±0.16
Qiagen	StepOnePlus	17.19±0.23	20.05±0.27	24.09±0.57	27.23±0.93	31.02±0.69	33.67±0.35
	CFX Connect	17.08±0.26	20.81±0.06	22.81±0.13	26.62±0.32	30.36±0.45	33.36±0.17
Takara	StepOnePlus	18.69±0.37	22.00±0.45	26.47±0.18	29.91±0.69	33.28±0.21	ND
	CFX Connect	16.68±0.18	19.65±0.25	22.77±0.34	26.24±0.23	29.02±0.21	ND

Average C_T values (mean±SD) for 50, 5, 0.5, 0.05, 0.005, and 0.0005 ng/μL porcine DNA analyzed in triplicate.
ND, not detected.