Table 3. Comparison of the specificity of the seven commercial master mixes for detecting porcine DNA by real-time PCR
| Manufacturer | Platform | CT values1,2) of animal species |
| Pig | Human | Dog | Cow | Chicken |
| Applied Biosystems | StepOnePlus | 17.77±0.53 | ND | ND | ND | ND |
| CFX Connect | 17.38±0.23 | ND | ND | ND | ND |
| CancerROP | StepOnePlus | 18.54±0.09 | 33.21±0.54 | ND | 34.28 (2/3) | ND |
| CFX Connect | 15.72±0.44 | 34.11 (2/3) | ND | ND | ND |
| Invitrogen | StepOnePlus | 17.01±0.16 | ND | ND | ND | ND |
| CFX Connect | 17.60±0.34 | ND | ND | 34.28 (2/3) | ND |
| Kogene Biotech | StepOnePlus | 15.35±0.37 | ND | ND | ND | ND |
| CFX Connect | 17.40±0.45 | ND | ND | ND | ND |
| New England Biolabs | StepOnePlus | 17.27±0.04 | ND | ND | ND | ND |
| CFX Connect | 17.61±0.11 | 33.56±0.83 | ND | 34.91 (2/3) | ND |
| Qiagen | StepOnePlus | 17.19±0.23 | 33.96 (2/3) | ND | ND | ND |
| CFX Connect | 17.08±0.26 | 34.34 (2/3) | 32.75±0.73 | 33.92±0.33 | ND |
| Takara | StepOnePlus | 18.69±0.37 | ND | ND | ND | ND |
| CFX Connect | 16.68±0.18 | ND | ND | ND | ND |
Average CT values (mean±SD) for 50 ng/μL DNA samples of five species analyzed in triplicate.
Numbers in parentheses indicates the number of times the CT value was determined in three measurements, the average CT value is presented.
ND, not detected.