Table 5. Comparison of the time and cost associated with the seven commercial master mixes included in this study

Manufacturer	Master mix	Cost/reaction (US$)1)	Total time (min)2)
Applied Biosystems	TaqMan Universal PCR Master Mix	1.13	38.25
CancerROP	MG 2X qPCR MasterMix (TaqMan) with ROX	0.33	57.50
Invitrogen	Express qPCR Supermix Universal	1.26	47.75
Kogene Biotech	PowerAmp Real-time PCR Master Mix II	0.59	55.75
New England Biolabs	Luna Universal Probe qPCR Master Mix	0.47	27.25
Qiagen	QuantiNova Probe PCR Kit	0.85	10.75
Takara	Premix Ex Taq (Probe qPCR), ROX plus	0.49	12.58

Calculated based on the cost for each commercial master mix in spring 2020.
Summing of the cycling times in the manufacturer’s protocol.