Table 4. Production of diacetyl with the addition of different concentrations of citrate

Citrate (%, w/v) Diacetyl (mg/L)
0 19.85±0.38
0.1 40.48±0.32b
0.2 43.26±0.44b
0.3 42.75±1.41b
0.5 43.01±0.59b
1.0 42.85±1.43b
2.0 34.43±1.64a
3.0 35.20±1.05a
Results are expressed as mean±SE (n=3).
MRS broth was used as the basal medium, citrate was added before sterilization (autoclave, 121°C, 15 min). The initial concentration of diacetyl was 0.
Means in the same column with different lowercase superscript letters are significantly different at p<0.05.