| Instrument | Condition |
|---|---|
| Column | Hiprep 16/60 Sephacryl S-100 HR column (GE Healthcare Life Sciences) |
| Mobile phase | 5 mM sodium phosphate buffer (0.15 M NaCl, pH 7.0) |
| Detector (Detection) | Waters Dual λ Absorbance Detector W2487 (280 nm) |
| Flow rate | 1 mL/min |
| Injection volume | 3 mL |