Table 3. Biofilm-formation ability of Kocuria salsicia isolates (KS1–KS9) in nutrient broth (NB) containing 15% sodium chloride (NaCl) compared with that of Staphylococcus aureus ATCC 29213 at 5°C, 15°C, 25°C, 30°C, 37°C, and 42°C

Strains	Optical density of isolates¹⁾
	Temperature (°C)²⁾
	5	15	25	30	37	42
NC	0.040±0.000	0.042±0.001	0.040±0.000	0.045±0.002	0.045±0.000	0.045±0.000
PC	0.047±0.001^a	0.049±0.000^a	0.053±0.001^a	0.051±0.001^a	0.066±0.000^a	0.066±0.000^a
KS1	0.056±0.003^b	0.062±0.001^b	0.079±0.010^b	0.076±0.006^b	0.071±0.014^a	0.064±0.006^a
KS2	0.051±0.000^a	0.054±0.001^b	0.054±0.000^a	0.084±0.002^b	0.066±0.007^a	0.076±0.009^a
KS3	0.057±0.002^b	0.063±0.002^b	0.067±0.001^b	0.096±0.004^b	0.077±0.004^a	0.071±0.004^a
KS4	0.052±0.001^a	0.055±0.001^b	0.056±0.002^a	0.067±0.000^b	0.056±0.004^a	0.054±0.005^a
KS5	0.055±0.002^b	0.055±0.001^b	0.054±0.001^a	0.065±0.003^a	0.059±0.002^a	0.058±0.004^a
KS6	0.061±0.007^b	0.057±0.000^b	0.059±0.001^a	0.069±0.005^b	0.065±0.007^a	0.064±0.007^a
KS7	0.054±0.001^a	0.054±0.001^b	0.054±0.001^a	0.062±0.008^a	0.055±0.006^a	0.063±0.006^a
KS8	0.053±0.000^a	0.066±0.001^b	0.059±0.001^a	0.080±0.000^b	0.070±0.004^a	0.068±0.002^a
KS9	0.054±0.001^a	0.059±0.002^b	0.053±0.001^a	0.083±0.011^b	0.076±0.001^a	0.066±0.005^a

Optical density of isolates is expressed as mean±SD.
Biofilm formation by temperature (°C).
Different letters indicate statistical difference at p<0.05 compared to S. aureus ATCC 29213 (Tukey method).
ATCC, American Type Culture Collection; NC, negative control (NB containing 15% NaCl); PC, positive control (S. aureus ATCC 29213+NB containing 15% NaCl); KS1–KS9, K. salsicia KS1–KS9+NB containing 15% NaCl.