Table 2. Assignment of 1H- nuclear magnetic resonance chemical shifts of fraction EGCI 1-1, 1-2, and 1-3 separated from EGC (EGCI-1)

Reporter group Residue Chemical shifts, δ (coupling constants, Hz)
EGCI 1-1 EGCI 1-2 EGCI 1-3
H-1 Glc α 5.222 (4.0) 5.225 (3.4) 5.227 (3.4)
Glc β 4.663 (8.0) 4.669 (8.0) 4.671 (8.0)
Galβ4 4.530 (8.0) 4.427 (8.0) 4.431 (7.9)
H-3ax Neu5Ac(α2-3) 1.800 (12.01), −12.02)) - -
Neu5Ac(α2-6) - 1.743 (12.61), −12.02)) -
Neu5Gc(α2-6) - 1.762 (12.61), −12.02))
H-3eq Neu5Ac(α2-3) 2.756 (4.63)) - -
Neu5Ac(α2-6) - 2.711 (4.63)) -
Neu5Gc(α2-6) - - 2.731 (4.63))
NAc Neu5Ac(α2-3) 2.030 - -
Neu5Ac(α2-6) - 2.028 -
NGc Neu5Gc(α2-6) - - 4.118
J3ax, 4,
J3ax, 3eq,
J3eq, 4.
EGC, Etawah Grade colostrum.