Table 4. The range analysis of flavourzyme on DH obtained from the L9 (34) orthogonal experiment

No. (A) (B) (C) (D) DH (%)
1 1 1 1 1 42.64
2 1 2 2 2 40.98
3 1 3 3 3 32.47
4 2 1 2 3 46.76
5 2 2 3 1 34.65
6 2 3 1 2 35.34
7 3 1 3 2 35.96
8 3 2 1 3 33.56
9 3 3 2 1 43.45
K1 38.70 41.79 37.18 40.25
K2 38.92 36.4 43.73 37.43
K3 37.66 37.09 34.36 37.60
Best level A2 B1 C2 D1
R 1.26 5.39 9.37 2.65
R order C>B>D>A
Four controllable variables, including time (A), temperature (B), pH (C), and the amount of flavourzyme added (D) were selected for optimization.
DH, degree of hydrolysis.