Table 1. Absolute numbers of immune cells before or after treatment of cyclophosphamide (CPP)

Tissue	Population	Surface phenotype	Vehicle treatment	CPP treatment	p-value
Thymus (×106)	Total cell		246.67±14.70	5.83±2.03	<0.001
	DN	CD4–CD8–	9.87±1.39	0.40±0.15	0.002
	DP	CD4+CD8+	211.56±12.01	0.29±0.11	<0.001
	CD4+ SP	CD4+CD8–	18.62±1.13	3.68±1.28	0.001
	CD8+ SP	CD4–CD8+	5.99±0.54	1.41±0.47	0.003
Spleen (×106)	Total cell		50.17±0.73	11.00±3.12	<0.001
	T cell	CD3ε+	17.26±0.95	5.98±1.65	0.004
	CD4+ T cell	CD3ε+CD4+	9.07±0.40	2.64±0.73	0.002
	CD8+ T cell	CD3ε+CD8+	6.31±0.36	2.77±0.79	0.015
	B cell	B220+	25.51±0.73	2.93±0.90	<0.001
	NK cell	CD3ε–NK1.1+	1.38±0.15	0.12±0.04	0.001

All data represent the mean±SEM (n=3).
Significant differences were determined by p-values.
Vehicle, PBS treatment; DN, double negative cell; DP, double positive cell; SP, single positive cell; PBS, phosphate buffered saline.