Table 2. Susceptibility to antibiotics and enzyme activity of Lactobacillus plantarum KC3

Anti-microbial agents Minimal inhibitory concentration (μg/mL) EFSA suggested values Enzyme KC3
Amikacin 4 - Alkaline phosphatase 01)
Gentamycin 1 16 Esterase (C4) 0
Kanamycin 16 64 Esterase lipase (C8) 1
Streptomycin 8 - Lipase (C14) 0
Ampicillin 256 2 Leucine arylamidase 5
Penicillin-G 1 - Valine arylamidase 4
Oxacillin 8 - Cystinearylamidase 2
Bacitracin 32 - Trypsin 0
Polymyxin B 128 - α-Chymotrypsin 0
Ciprofloxacin 8 - Acid phosphatase 2
Tetracycline 16 32 Naphtol-AS-BI-phosphohydrolase 2
Clindamycin 0.0156 2 α-Galactosidase 0
Erythromycin 0.125 1 β-Galactosidase 5
Rifampicin 1 - β-Glucuronidase 0
Vancomycin 2,048 - α-Glucosidase 3
Choloramphenicol 4 8 β-Glucosidase 4
N-Acetyl-β-glucosaminidase 3
α-Mannosidase 0
α-Fucosidase 0
A value ranging from 0 to 5 is assigned to the standard color: Zero represents a negative; 5 represents a reaction of maximum intensity. Values 1 to 5 represent intermediate reactions depending on the level of intensity. The approximate activity may be estimated from the color strength: 1 Corresponds to the liberation of 5 nanomoles, 2 to 10 nanomoles, 3 to 20 nanomoles, 4 to 30 nanomoles, and 5 to 40 nanomoles or more.
EFSA, European Food Safety Authority.