Table 3. Effects of pink inhibiting ingredients without added STP, cooking rate1), and storage day2) on Mb content, total pigments, PMD, rNIC, and nitrosyl hemochrome in cooked ground turkey breast

Main effects Mb (uncooked, mg/g) Total pigments (ppm) PMD (%) rNIC Nitrosyl hemochrome (ppm)
Slow Fast Day 1 Day 14
Treatment (T)3)
 Control 0.762a 18.24d 84.3bcx 85.9bcx 1.026bx 1.004aby 0.38cd
 CA 0.1% 0.753ab 22.12c 86.2abx 86.6abcx 1.012dx 1.001by 0.40bcd
 CA 0.2% 0.708ab 25.22b 84.9bcy 87.3abx 1.003ex 0.998cy 0.46abcd
 CA 0.3% 0.598c 27.97a 81.7dy 86.2bcx 0.993fx 0.991dx 0.52ab
 CC 0.025% 0.738ab 18.20d 83.9cx 85.3cx 1.021cx 1.003by 0.35d
 CC 0.05% 0.759a 18.26d 83.6cy 86.0bcx 1.020cx 1.004aby 0.38cd
 EDTA 0.005% 0.696b 22.66c 87.2ax 86.0bcx 1.027abx 1.006aby 0.49abc
 EDTA 0.01% 0.762a 23.09c 87.2ax 87.9ax 1.020cx 1.005aby 0.53a
 SC 0.5% 0.744ab 22.28c 84.9bcy 86.9abcx 1.027abx 1.006aby 0.42abcd
 SC 1.0 % 0.695b 22.32c 84.2cy 86.8abcx 1.031ax 1.008ay 0.36d
 (SEM) (0.057) (0.83) (1.57) (0.002) (0.05)
Cooking rate: The samples were cooked by loading the tubes into a 50°C water bath and then immediately setting the water bath to 90°C (slow cooking) or cooked to 76.7°C in a 90°C water bath (fast cooking).
Storage day: Samples were stored (2°C to 3°C) in the dark before color was determined.
Treatment: Ground meat was salted (2% NaCl) and stored for 6 days before pink inhibiting ingredients (PII: Control=no added PII; CA, CC, EDTA, SC) were incorporated, and then stored overnight prior to cooking.
Means within a column with unlike superscript letters are different (p<0.05). Significant treatment by cooking rate interaction for PMD (p<0.05). Significant treatment by storage day interaction for rNIC (p<0.0001).
Means under PMD and rNIC interaction effects within a row with unlike superscript letters are different (p<0.05).
STP, sodium tripolyphosphate; Mb, amount of undenatured myoglobin; PMD, percentage myoglobin denaturation; rNIC, reflectance estimator of nicotinamide hemochrome (%R537 nm/%R553 nm); CA, citric acid; CC, calcium chloride; EDTA, ethylenedinitrilotetraacetic acid disodium salt; SC, sodium citrate; PII, pink inhibiting ligands.