Table 1. Gas chromatography operating conditions for determination of horse oil fatty acid composition

Item	Condition
Instrument	Agilent 6890N (Agilent Technologies, Palo Alto, CA, USA)
Column	Omegawax250 (30 m×0.25 mm id, 0.25 μm film thickness; Supelco, Bellefonte, PA, USA)
Detector	Flame ionization detector
Carrier gas	Helium (99.99%, research purity)
Column flow rate	1.2 mL/min
Split ratio	100:1, 1 μL (injection volume)
Injection port temperature	250°C
Detection port temperature	260°C
Oven temperature	150°C, hold for 2 min4°C/min up to 220°C, hold for 30 min