Table 3. The molecular weight distribution of peptide extracts (<10 kDa) from Chickso, Hanwoo, and Wagyu shank
Contents | Chickso | Hanwoo | Wagyu |
Before digestion | After digestion | Before digestion | After digestion | Before digestion | After digestion |
Mn (Da) | 508±15.54A | 285±6.19B | 324±5.84B | 296±4.42B | 534±10.11A | 280±7.38B |
Mw (Da) | 1,954±59.62A | 395±9.27B | 527±9.69B | 435±8.53B | 2,136±76.19A | 325±9.32B |
Mz (Da) | 4,821±73.83A | 730±11.25B | 901±11.38B | 799±10.52B | 5,268±112.38A | 381±15.25C |
Polydispersity | 3.85±0.12A | 1.39±0.08B | 1.63±0.11B | 1.47±0.11B | 4.00±0.20A | 1.16±0.09 |
>10,000 Da (%) | 1.63±0.05 | - | - | - | 2.05±0.04 | - |
5,000–10,000 Da (%) | 7.86±0.78 | - | - | - | 10.28±0.15 | - |
3,000–5,000 Da (%) | 12.74±0.88 | 0.18±0.01 | 0.11±0.01 | 0.15±0.01 | 12.36±0.38 | - |
1,000–3,000 Da (%) | 26.65±1.25 | 5.36±0.08 | 12.98±0.35 | 7.74±0.86 | 24.48±2.54 | - |
200–1,000 Da (%) | 39.83±3.12 | 80.43±1.49 | 69.99±2.89 | 77.30±3.98 | 41.35±3.15 | 85.70±1.17 |
<200 Da (%) | 11.30±0.52 | 14.03±0.28 | 16.93±1.12 | 14.81±0.95 | 9.49±0.49 | 14.30±0.28 |
Each value is a mean±SD of three replicates.
Different uppercase letters in the same line indicate significant differences among Chickso, Hanwoo, and Wagyu shank peptides (p<0.05).
Mn, number-average molecular weight; Mw, weight-average molecular weight; Mz, Z-average molecular weight.