Table 4. Changes in the amino acid compositions of Chickso, Hanwoo, and Wagyu loin peptides due to in vitro digestion

Amino acids (mg/100 g)	Chickso		Hanwoo		Wagyu
Amino acids (mg/100 g)	Before digestion	After digestion	Before digestion	After digestion	Before digestion	After digestion
Aspartic acid	217.2±12.5^B	236.7±15.1^A	205.3±11.2^B	232.8±19.8^A	187.5±15.5^C	166.7±11.5^D
Threonine	100.0±3.8^B	416.2±21.9^A	103.7±5.9^B	408.8±28.7^A	109.8±9.6^B	467.1±15.3^A
Serine	88.6±2.4^C	280.0±15.8^A	90.8±3.8^C	275.9±12.7^A	80.1±4.2^C	151.4±11.5^B
Asparagine	74.7±2.3^C	290.8±13.7^A	89.5±4.1^C	287.9±22.3^A	65.3±5.8^C	172.3±12.7^B
Glutamic acid	947.1±52.1^C	1,133.3±95.3^A	940.2±22.7^C	1,113.7±75.1^A	828.4±41.7^D	1,024.1±83.4^B
Glutamine	10.8±0.7^C	450.9±41.1^B	12.4±0.9^C	437.4±33.5^B	9.9±0.3^C	689.3±22.3^A
Glycine	39.4±1.5^C	326.9±15.8^A	38.6±0.8^C	326.2±18.9^A	31.5±2.1^C	201.6±17.2^B
Alanine	164.7±12.2^B	529.7±29.2^A	167.7±11.5^B	521.5±37.8^A	146.5±10.9^B	498.9±18.9^A
Valine	176.1±13.6^C	495.2±22.5^A	177.0±14.9^C	488.3±16.5^A	169.7±13.2^C	392.2±26.3^B
Cystine	80.8±5.4^B	140.4±13.1^A	82.6±4.3^B	145.4±11.2^A	51.0±2.3^B	136.8±11.2^A
Methionine	259.5±12.9^B	805.8±42.3^A	260.1±15.7^B	790.1±38.9^A	211.2±15.8^B	673.1±45.7^A
Isoleucine	291.3±15.8^C	690.0±44.8^A	288.4±24.4^C	683.6±101.2^A	278.3±21.2^C	549.6±15.4^B
Leucine	1,067.9±98.5^C	3,279.8±239.8^A	1,067.0±82.3^C	3,212.2±284.5^A	854.8±33.6^C	2,366.4±115.7^B
Tyrosine	158.7±10.6^C	1,754.5±112.7^B	138.4±8.8^C	1,717.1±211.7^B	164.2±17.7^C	2,041.8±132.4^A
Phenylalanine	182.5±11.2^C	1,815.2±89.5^A	189.2±13.1^C	1,781.7±189.4^A	178.0±11.5^C	1,551.2±109.8^B
Tryptophan	89.5±8.3^C	293.3±19.8^A	81.4±3.8^C	274.2±42.1^A	151.3±8.0^B	292.8±12.1^A
Proline	44.1±6.9^B	93.0±5.3^A	37.0±2.6^B	91.5±15.3^A	24.4±3.2^C	95.7±3.8^A
Lysine	394.1±27.3^C	2,651.1±127.7^A	391.5±24.3^C	2,591.4±306.2^A	498.7±11.7^B	2,606.6±201.7^A
Histidine	28.3±2.2^C	278.3±12.6^B	28.3±1.6^C	278.4±17.6^B	33.8±1.9^C	356.3±16.9^A
Arginine	291.2±11.8^C	2,886.6±227.9^A	292.8±17.5^C	2,838.4±165.9^A	250.7±12.1^C	1,560.8±137.0^B

Each value is a mean±SD of three replicates.
Different uppercase letters in the same line indicate significant differences among Chickso, Hanwoo, and Wagyu loin peptides (p<0.05).