Table 4. Free amino acid profile (mg/kg) of yeast protein after hydrolysis treatment with different enzymes (endo- and exotype)

Free amino acids	Control	Endo protease		Exo protease
Free amino acids	Control	Alcalase	Neutrase	Flavourzyme	Prozyme 2000P
Aspartic acid	15.10±0.03^d	799.84±63.91^c	69.36±1.96^d	10,980.45±112.41^a	5,981.38±182.86^b
Glutamic acid	42.19±0.68^d	2,341.31±139.60^c	99.25±1.72^d	15,045.57±164.85^a	7,317.84±167.81^b
Asparagine	1.32±0.19^d	593.82±26.01^c	54.89±2.58^d	10,875.72±116.03^a	8,017.27±216.41^b
Serine	4.21±0.04^d	606.73±34.73^c	120.14±2.58^d	13,716.34±121.55^a	9,928.50±255.77^b
Glutamine	2.52±0.13^d	166.40±9.62^c	33.96±2.29^d	9,184.48±90.40^a	6,156.48±163.99^b
Histidine	3.87±0.09^c	112.77±7.16^c	56.69±4.41^c	7,584.09±156.37^a	6,857.71±275.53^b
Glycine	7.51±0.22^d	138.33±10.83^c	40.43±1.49^d	5,736.49±16.02^a	3,095.87±76.12^b
Threonine	2.72±0.13^c	291.24±15.23^c	89.03±5.14^c	15,445.25±162.29^a	14,161.13±388.80^b
Citrulline	3.38±0.08^b	7.17±0.39^b	8.27±0.25^b	52.68±7.38^a	52.47±1.01^a
Arginine	11.24±0.17^b	260.00±9.32^b	160.59±8.17^b	21,116.10±144.10^a	20,836.41±390.38^a
Alanine	22.52±0.23^e	755.01±35.98^c	378.09±2.84^d	15,255.88±102.09^a	11,158.88±241.07^b
Tyrosine	16.13±0.20^c	680.82±20.57^c	248.60±5.95^c	16,061.61±422.56^a	13,553.08±457.60^b
Valine	5.01±0.25^d	428.94±16.73^cd	662.75±7.53^c	20,025.00±258.62^a	18,670.87±430.27^b
Methionine	2.33±0.45^c	434.37±17.49^b	261.73±7.36^b	6,401.54±75.83^a	6,527.38±163.48^a
Tryptophane	20.17±0.64^c	104.79±9.93^c	ND	3,586.82±56.27^a	3,432.12±104.89^b
Phenyl-alanine	18.61±0.48^d	786.07±22.89^c	916.04±11.68^c	19,174.72±242.16^b	21,442.14±564.94^a
Isoleucine	1.56±0.22^b	163.72±41.32^b	294.17±4.40^b	16,431.76±191.37^a	16,143.14±368.14^a
Leucine	2.36±0.21^d	1,946.93±83.95^c	1,413.05±31.50^c	32,462.11±419.81^b	34,292.80±862.93^a
Lysine	117.01±3.72^c	543.53±30.93^c	325.51±28.62^c	30,678.46±183.12^a	23,492.51±445.84^b
Proline	14.15±0.36^b	ND	ND	1,091.38±13.04^a	935.35±152.86^a
AAAs	54.91±0.32^b	1,571.68±32.02^b	1,164.65±14.07^b	38,823.15±716.29^a	38,427.33±1,119.54^a
HAAs	80.33±1.12^c	4,545.64±178.91^b	3,796.34±55.57^bc	115,234.95±1,651.70^a	114,996.88±3,067.61^a
EAAs	171.32±3.05^c	4,377.98±195.83^c	3,757.24±78.70^c	145,388.22±1,165.98^a	138,492.41±3,401.26^b
TAAs	313.92±3.07^d	11,161.78±553.29^c	5,232.57±93.61^cd	270,906.45±2,484.28^a	232,053.32±5,810.07^b
EAAs/TAA (%)	54.57±0.50^c	39.23±0.25^e	71.80±0.48^a	53.67±0.09^d	59.68±0.04^b

Data are expressed as mean±SD (n=3).
The means indicated with different letters within the same row are significantly different at p<0.05.
AAAs, aromatic amino acids; HAAs, hydrophobic amino acids; EAAs, essential amino acids; TAAs, total amino acids; ND, not detected.