Table 1. Multiple reaction monitoring (MRM) conditions

Target	Retention time (min)	Ionization	Exact mass (g/mol)	Precursor ion (m/z)	Product ion (m/z)	Collision energy (eV)
Flunixin	7.19	[M+H]+	296.1	297.1	279.0, 263.9, 281.0	22, 33, 14
5-Hydroxy flunixin	6.78	[M+H]+	312.2	312.9	279.9, 295.0	34, 23