Bacillus cereus | Sample 25 g/0.1%, peptone water 225 mL, 121°C, 15 min | MYP agar base 21.5 g/450 mL, 121°C, 15 min, polymyxin B supplement 1 vial, egg yolk emulsion 50 mL |
Clostridium perfrigens | Cooked meat 1 g/10 mL, 121°C, 15 min, sample solution (0.1% PW) 1 mL/10 mL | TSC agar [perfringens agar base (TSC/SFP)]23 g/500 mL, 121°C, 10 min, perfringens (TSC) supplement 1 vial, egg yolk emulsion 25 mL |
Staphylococcus aureus | Sample 25 g/225 mL, Tryptone Soya Broth(CM0129, Oxoid, Basingstoke, UK) 30 g/L, NaCl 10 g/L, 121°C, 15 min | Baird-Parker Agar Base (CM0275, Oxoid)31.5 g/500 mL, Egg Yolk Tellurite Emulsion (SR0054C, Oxoid) 25 mL, 121°C, 15 min |
Listeria monocytogenes | Sample 25 g/225 mL,Listeria Enrichment Broth (CM0862, Oxoid) 36 g/L, Listeria Selective Enrichment Supplement (SR0141E, Oxoid) 2 vial/L, 121°C, 15 min | PALCAM Agar Base (CM0877B, Oxoid)34.5 g/500 mL, PALCAM selective supplement (SR0150E, Oxoid) 1 vial, 121°C, 15 min |
Fraser broth 0.1 mL/10 mL,Fraser Broth Base (CM0895, Oxoid) 28.7 g/500 mL,Fraser Broth Supplement (SR0156, Oxoid) 1 vial,121°C, 15 min |
Escherichia coli O157:H7 | Sample 25 g/225 mL, mTSB broth (modified tryptic soy)33 g/L, norobiocin supplement, 121°C, 15 min | TC-SMAC (sorbitol MacConkey agar)25.75 g/500 mL, 121°C, 15 min, cefixime tellurite selective supplement 1 vial |
Salmonella spp. | Buffered peptone water 20 g/L, 121°C, 15 min, sample 25 g/225 mL | XLT4 agar (XLT4 agar) 2,935 mg/500 mL,XLT4 supplement 2.3 mL, boil for 1 min |
RV [rappaport-vassiliadis (RV) enrichment broth]15 g/500 mL, 115°C, 15 min, sample solution 0.1 mL/10 mL |