Table 4. Overview of analytical methods and technologies for detecting contamination

Non-compliance concern Meat/meat product Method/technology Summary of findings Reference
Contamination Pork contamination in halal beef and goat sausages Densitometry analysis - Actin fraction <50 kDa) identified as a potential biomarker for detecting pork in processed meat products.- Precision and accuracy tests (KV<5%, percent recovery>95%) confirmed the method’s effectiveness in testing halalness, particularly for pork-contaminated sausages. Hermanto et al. (2022)
Six prohibited meats (donkey, cat, pig, rat, dog, and monkey) contamination in halal beef meatballs and other commercial food products High resolution melting analysis (HRMA) - Prohibited animal DNA limit of detection: 0.01 ng (except pig DNA, which is 0.001 ng).- Method achieved 100% accuracy in identifying intentionally adulterated non-halal meats in beef meatballs.- Method validation with 260 Thai food products identified two samples contaminated with pig DNA. Denyingyhot et al. (2021)
Porcine blood contamination Monoclonal antibodies (MAbs) - Qualitative ELISA characterized MAbs against blood, non-blood, and plasma from different species.- Twelve MAbs exhibited specificity to porcine plasma.- MAbs recognizing 60 kDa heat-treated soluble proteins in porcine blood and plasma were selected as a novel approach for detecting porcine plasma in processed food. Raja Nhari et al. (2023)
Pork contamination in beef extract Molecularly imprinted polymer nanogel (MIP-NG)-based sensor - Fluorescent molecularly imprinted polymer nanogel (F-MIP-NG) sensor exhibited excellent analytical performance to detect porcine serum albumin.- Rapid detection, less than 5 minutes per sample.- Low detection limit of 0.1 wt% for pork contamination. Cheubong et al. (2021)
Porcine contamination Interdigitated electrode (IDE) - Titanium dioxide (TiO2) deposition on IDEs for optimization.- IDE could detect porcine presence at 1.0 μM.- Gold replacement may enhance device sensitivity. Nordin et al. (2016)