| 1 | Scale up economics for cultured meat | Humbird (2021) | Economic challenges and microbial risks in scaling cultured meat production | Scalability is limited by low growth rates, metabolic inefficiency, and cell damage. Meeting the target cost of ~$25/kg for bulk cell mass is essential, though perfusion processes exceed this cost. Plant hydrolysates may offer a solution but require further development. Enhancing metabolic efficiency and lowering media costs are crucial for displacing conventional meat. |
| 2 | Microbiological and chemical hazards in cultured meat and methods for their detection | Sogore et al. (2024) | Examination of microbial and chemical hazards in cultured meat production | Identified potential microbial and chemical contaminants at each stage of production. Emphasized the need for robust safety protocols, scalable testing methods, and specialized detection systems to monitor contaminants unique to cultured meat. Recommended implementing digital food safety technologies for real-time monitoring and regulation to enhance safety and consumer confidence. |
| 3 | Challenges and possibilities for bio-manufacturing cultured meat | Zhang et al. (2020) | Examination of technological and societal challenges in cultured meat production | Identified challenges including high production costs, lack of nutrients, and the need for food safety certification. Proposed solutions involve advancements in tissue and bioreactor engineering, synthetic biology, and the development of serum-free culture media. Public acceptance remains a hurdle, with concerns over scalability, cost, and safety. |
| 4 | Quality and Risk Control in Cultivated Meat Production Check for updates | de Macedo et al. (2024) | Quality control and risk assessment in cultivated meat production | Emphasized the importance of reproducibility, donor selection, and cell culture safety in cultivated meat production. Identified the need for strict microbiological controls, environmental monitoring, and addressing risks like heavy metals, toxins, and pathogens. Highlighted the significance of regulatory frameworks to ensure consumer trust and safety in the final product. |
| 5 | Biotechnological and technical challenges related to cultured meat production | Lanzoni et al. (2022) | Challenges and approaches for large-scale cultured meat production | Identified key steps and challenges in cultured meat production, such as animal selection, FBS alternatives, and scalable biofabrication systems. Biotechnological hurdles include replicating traditional meat’s nutritional and functional quality, while technological challenges focus on optimizing scaffolding, 3D bioprinting, and bioreactors for large-scale production. |
| 6 | Considerations for the development of cost-effective cell culture media for cultivated meat production | O’Neill et al. (2021) | Challenges in designing cost-effective culture media for large-scale production | This study focuses on the critical role of culture media in cultivated meat production. It highlights the need for developing affordable, food-grade, and animal-ingredient-free media to support large-scale muscle cell proliferation and differentiation. Drawing insights from conventional culture media applications and microbial fermentation processes, it emphasizes that overcoming media-related challenges will be crucial for successful commercialization of cultivated meat. |