Table 1. Effect of ultrasound to inactivate microorganism in animal-based food

Food product Treatment type Ultrasound specification Process conditions Affected microorganisms Microorganisms’ inactivation References
Meat
 Chicken breasts Ultrasound - Frequency: 40 kHz- Intensity: 9.6 W/cm2 - Ultrasound applied time: 0, 30, and 50 min- Storage: aerobic and anaerobic/48 h at 4°C - Psychrophilic bacteria- Staphylococcus aureus - S. aureus significant reduction after 50 min ultrasound treatment- Psychrophilic bacteria significant reduction under anaerobic packaging Piñon et al. (2020)
 Chicken skin Ultrasound and ethanol treatment combination - Frequency: 37 kHz- Power: 380 W - Ultrasound applied for 5 min- Ethanol (30%, 50%, 70%) - Mesophilic aerobic bacteria (MAB)- Coliforms- Salmonella Typhimurium - In combination of 30% ethanol with ultrasound reduced S. Typhimurium by >1.0 Log CFU/g- Ethanol (30% or 50%) with ultrasound significantly reduced MAB 1.38–2.60 Log CFU/g and for coliforms 1.04 to 1.80 Log CFU/g Seo et al. (2019)
 Dry-cured ham Supercritical carbon dioxide (SC-CO2), saline (SS), and high-power ultrasound (HPU) combination - Frequency: 30 kHz- Power: 40±5 W - Pressure: 150–350 bar- Temperatures: 41°C–51°C- Time: 5–15 min - Escherichia coli - For maximum inactivation optimal conditions are;· (SC–CO?+HPU+SS):48.4°C, 12.2 min and 243.3 bar → 3.88 Log reduction· (SC–CO?+HPU):51°C, 5 min and 350 bar→ 3.88 Log reduction Castillo-Zamudio et al. (2021)
 Bovine loins Ultrasound - Frequency: 37 kHz- Intensities: 16, 28, 90 W/cm2- Ultrasonic baths models: S15H, S40H, and S60H - Sonication time: 20 and 40 min- Storage time: 0 and 7 days at 4°C - MAB- Psychrophilic bacteria- Coliform - Ultrasound at 90 W/cm2 effective in controlling- MAB and psychrophilic bacteria during storage at 4°C- At a sonication time of 40 min, coliform was reduced by ultrasound regardless of intensity Carrillo-Lopez et al. (2019)
 Pork jowl Steam-ultrasound - Frequency: 30–40 kHz - Steam at 130°C and 3.5–5 atm pressure- Time: 0, 1.0, 2.0, 3.0, or 4.0 sec - Yersinia enterocolitica- S. Typhimurium- E. coli - 0.5 s treatment had higher reductions of 0.9–1.5 Log CFU/cm2 for E. coli compared to 0.4–1.1 Log CFU/cm2 for S. Typhimurium and Y. enterocolitica Morild et al. (2011)
 Pork Ultrasound combination - Frequency: 25 kHz and power 300 W and 1 MHz with power 150 W - Time: 10 min- Temperature: 12°C - Campylobacter jejuni- Brochothrix thermosphacta- Listeria monocytogenes - Ultrasound combined with red wine showed higher bacterial reductions compared with ultrasound or red wine alone.- C. jejuni, L. monocytogenes, B. thermosphacta had significant reduction of more than 1 Log Birk and Knøchel (2009)
 Beef slurry Thermo-sonication - Frequency: 24 kHz- Intensity: 0.33 W/g- Amplitude: 100% - Temperature: 75°C- Time: 60 min - Clostridium perfringens NZRM 898 and NZRM 2621 spores- (NZRM 898 and NZRM 2621 refer to specific strains of C. perfringens spores) - At 75°C; thermo-sonication with 24 kHz ultrasound at 0.33 W/g achieved less than 1.5 Log reductions for both C. perfringens NZRM 898 and NZRM 2621 spores after 60 min Evelyn and Silva (2015)
Eggs
 Liquid whole egg Ultrasound, lysozyme, and heat combination - Power: 950 W- Power levels of 50% (605 W/cm2) and 80% (968 W/cm2) - Ultrasound and Lysozyme (US+Lys) combination treatment- Temperature: 35°C,- Time: 20 min - S. Typhimurium - Best microbial inactivation by ultrasound and lysozyme (US+Lys) → 4.26 Log reduction Bi et al. (2020)
 Liquid whole eggs Hugh intensity ultrasound (HIU) - Frequency: 20 kHz- Amplitude: 80% - HIU treatment duration: 1, 5, 10, and 30 min (pulsed intervals: 30 s on/off)- Temperature: 20°C - Salmonella Enteritidis - HIU 1 min → significant reduction of 1.9 Log CFU/mL Techathuvanan and D'Souza (2018)
 Liquid whole egg Hydraulic high pressure (HHP), ultrasound (US), and pulsed electric field (PEF) - Frequency: 20 kHz- Power 40 W - (HHP+US) combination· US: 5 min/55°C· HHP: 2–2–4 min (cyclic treatment) at 138 MPa, 20°C - S. Enteritidis - Combination of HHP and ultrasound → highest reduction of S. Enteritidis (3.23 Log cycles) Huang et al. (2006)
 Japanese quail eggshell Ultrasound - Frequency: 35 kHz and 130 kHz - U35 (35 kHz): 30 min- U130 (130 kHz): 30 min - Coliform- Salmonella- Staphylococcus - U130 initially reduced coliform, Salmonella, and Staphylococcus counts on eggshells Yildirim et al. (2015)
Milk
 Cow milk Ultrasound - Frequency: 20 kHz- Intensity: 118 W/cm2- Power: 150 W- Amplitude: 100% - Batch ultrasound treatment: · Temperatures: 20±2°C and 57±2°C · Time: 1, 3, 4, and 6 min- Continuous flow ultrasound treatment:· Temperatures –20± 2°C and 57±2°C.· Time: 3, 9, 12, and 18 min - L. monocytogenes - Continuous flow ultrasound treatment:· UHT Milk with L. monocytogenes → 5 Log reduction· Raw milk total aerobic bacteria→ 5 Log reduction D’Amico et al. (2006)
 Goat milk Thermo-sonication - Frequency: 20 kHz- Power levels: 150 W, 200 W, 300 W, 400 W- Temperature: 72°C - Time: 10 min - MAB - At 400 W, thermosonication significantly reduced the microbial count to less than 2.3 Log CFU/mL, compared with 5.94 Log CFU/mL in raw goat milk and 4.76 Log CFU/mL in pasteurized goat milk Ragab et al. (2019)
 Cow milk Ultrasound - Frequency: 24 kHz- Power levels: 400 W- Acoustic power: 160.4 J/s- Amplitude: 70% and 100% - Time: 50, 100, 200, and 300 s - E. coli- S. aureus- Pseudomonas fluorescens- Debaryomyces hansenii - In the best treatment 100% amplitude for 300 s:· D. hansenii → 4.61 Log reduction· P. fluorescens → 2.75 Log reduction· E. coli → 2.09 Log reduction· S. aureus → 0.55 Log reduction Marchesini et al. (2015)
 Cow milk Thermo-ultra-sonication - Frequency: 20 kHz- Power: 1,500 W- Amplitude: 95%- Temperature: 45°C - Time: 10 or 15 min- Storage: 1, 7, and 14 days - MAB- Enterobacteria - MAB → within accepted range (except for homogenized, thermo-ultrasound 10 min)- Homogenized milk, thermo-ultrasound 15 min had the lowest Enterobacteriaceae count Hernández-Falcón et al. (2018)
 Whole milk and skimmed milk Ultrasound - Frequency: 24 kHz- Intensity: 85 W/cm2 - Temperature: 30°C–35°C - E. coli- L. monocytogenes - E. coli had significantly higher D values (Decimal reduction time) in:· Whole milk: 2.43 min· Skim milk: 2.41 min- L. monocytogenes also had significantly higher D values in:· Whole milk: 9.31 min· Skim milk: 8.61 min- E. coli showed log-linear inactivation followed by tailing, whereas L. monocytogenes exhibited 1st-order inactivation throughout- Ultrasound waves caused mechanical damage to the bacterial cell wall and membrane, leading to their inactivation Gera and Doores (2011)