Table 4. Cases of producing plant-based meat analogues using advanced processing technologies

Technology Pros and cons of technology Plant protein Subject References
High-moisture extrusion processing (HMEP) - Pros: Dense fibrous structure- Cons: Short shelf life due to high moisture content (Choi and Ryu, 2022) Pea protein, amylose, amylopectin Analyzing the effect of protein interactions with amylose and amylopectin on fiber structure Chen et al. (2022b)
Soy protein Compare morphological development of analog mittens as temperature changes Wittek et al. (2021)
Pea protein, peanut protein, soy protein, wheat gluten, rice protein Comparing the water-binding capacity of protein sources to improve the juiciness of analog meats Hu et al. (2024)
Pea protein isolate (PPI), pea protein concentrate (PPC) Compare the quality and organoleptic characteristics of PPI and PPC when mixed with ground beef. Pöri et al. (2023)
Soy protein, pea protein, wheat gluten Improve the texture of analog meat and provide quality control techniques Flory et al. (2023)
Low-moisture extrusion processing (LMEP), HMEP LMEP- Pros: Easy handling, long shelf life- Cons: Expanded structure with porous layers Soy protein, wheat gluten Comparison of analog mitt chemistry by LMEP and HMEP Choi and Ryu (2022)
Shear cell - Pros: Formation of fibrous structure- Cons: Testing is limited to laboratory scale (Krintiras et al., 2015) Soy protein, pea protein, wheat gluten Comparison of mixing and hydration effects of different protein sources and analysis of how mixing time affects the structure of analog meat Köllmann et al. (2024)
Soy protein, pea protein, wheat gluten Analyze the texture of analog meats made with shear cells with different strengths of vibration. Giménez-Ribes et al. (2024)
Soy protein Analyzing how the addition of salt affects the texture and structure of analog meat Dinani et al. (2023)
Ohmic heating - Pros: High efficiency in converting electrical energy into heat- Cons: Insufficient research on producing meat analogues (Jung et al., 2022) Soy protein, wheat gluten Analyzing the effect of cooking time and temperature on the texture and physicochemical properties of analog meat during ohmic heating Jung et al. (2022)
Peanut protein Confirming the effectiveness of ohmic heating as a technique to improve the structure and flavor of analog meat Chen et al. (2023)
Freeze structuring - Pros: Unique fibrous structure- Cons: High production costs due to high energy consumption (Du et al., 2023) Pea protein, wheat gluten Developing vegetable protein composites with improved nutritional and textural properties using cryostructuring technology Yuliarti et al. (2021)
Fiber-spinning - Pros: Micron-level protein fiber formation- Cons: High requirements for protein solutions, heavy contamination (Wang et al., 2023) Soy protein Developing soy protein-based analog meat with improved nutritional, physicochemical, and structural properties Joshi et al. (2023)
3D Printing - Pros: Control of fiber structure arrangement and distribution of adipose tissue- Cons: Plant-based meat analog inks are difficult to extrude, making it difficult to mimic the texture of animal meat Pea protein Rheology and extrusion testing to develop printable, print process-optimized formulations Wang et al. (2022a)
Mung bean protein, wheat gluten Improving the functionality of mung bean protein, wheat gluten mixtures, and adding L-cysteine to improve the quality and sensory characteristics of analog meat Chao et al. (2024); Wen et al. (2023)