Table 1. Primer sequences

Genes	Accession number	Sequence (5’–3’)	Product size (bp)
Pax7	XM_015460690	TGCCCTCAGTGAGTTCGATTCGGGTTCTGACTCCACATCT	180
GAPDH	NSO_4761240	ACTCTGGCAAAGTGGATGTTGTCGCATCACCCCACTTGATGTTG	95
PCNA	NM_001034494.1	GTCCAGGGCTCCATCTTGAAGAAAGGCTGCACCAAGGAGACATGAGAC	225
KI67	XM_015460791.2	CAGTCAACACGCCGACCAGTAAGCATCACCTGCTGCTTCTCCTTCTG	144
SLC23A2	NM_001205430.1	TCAGACAGAACCCTCTCGTCACAGAAAGCCACACAGCCTCCTACAAAC	97
CDK1	NM_174016.2	CTCGGTGTCCTACTTCAAGTGTGTGTCGCAGACCTCCAGCATCCAG	87
CDK2	NM_001014934.1	GACGGAGCTTGTTATCGCAAATGCGAGGTACTGGCTTGGTCACATCTTG	108
MyoD	NM_001040478	CCGACGGCATGATGGACTACTCGCTGTAGTAAGTGCGGT	80
MyoG	NM_001111325	CAGTGAATGCAGCTCCCATAGGCAGATGATCCCCTGGGTTG	87
MyHC	NM_001101835.1	GCTGGCTGGAGAAGAACAAGGACTGGTGAAGGTGGCGTAGAGGTG	102

Annealing temperature for all reactions is 60°C.
Pax7, paired box 7; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PCNA, proliferating cell nuclear antigen; KI67, tumor proliferation antigen; SLC23A2, nucleoside base transporter protein; CDK1, cell cycle protein-dependent kinase 1; CDK2, cell cycle protein-dependent kinase 2.