Effect of Modified Atmosphere Packaging Varying in CO₂ and N₂ Composition on Quality Characteristics of Dry Fermented Sausage during Refrigeration Storage

The prototype meat processing center at Daegu University’s Animal Resources Department produced low-temperature dry fermented sausages. Fresh pork lion was purchased from a commercial market in Geyongsan, Korea which were vacuum packaged. The back fat was thawed for 24 h at 4°C. The lean meat was preserved in the refrigerator for later use after cutting the connective tissues and extra fat. With the use of a 3–4 mm plate, chilled pork and pig fat were cut into small cubes and minced twice in a meat mincer (M-12S, Hankook fujee Industries, Suwon, Korea). Ground pork (65%), pig fat (21.5%), iced water (10%), NPS (97:3, a blend of sodium chloride and nitrite; 0.34%), NaCl (1.70%), sugar (0.45%), glucose (0.45%), sodium ascorbate (0.20%), and sausage seasonings (0.36%) are all included in the basic sausage formulation. For the start of the fermentation, one mL/kg (v/w) mixed starter cultures of Lactobacillus sakei and Staphylococcus xylosus were added and properly blended using a rotary slice cutter (SF-2002, Samwoo Industry, Daegu, Korea). Each starter culture had approximately 6 Log CFU/g, and the intended suspension put into the sausage batter was one mL/kg (v/w). With a vacuum filling machine (RVF 327, Düker-REX Fleischereimaschinen Gmbh, Laufach, Germany), the batter was filled into collagen casings (IKJIN, Seoul, Korea), 2.4 cm diameter and 15 cm length. The sausages were fermented and ripened in a digital chamber system with a temperature and relative humidity (RH) control unit (SMK-2000SL, Metatek, Seoul, Korea). The temperature was kept at 23°C for the first seven days of fermentation, and the RH was regulated between 90% to 95%. Following that, the ripening process was conducted for 28 days (following the fermentation process) at 15°C (the temperature was gradually reduced from 23°C), with RH varying between 70% to 75%.

After the completion of the ripening process, sausages were packed in their respective treatments as follows. Nylon/PE bags (80 μm thick: 15 μm for Nylon and 65 μm for PE) (Gasung Pak, Gwangju, Korea) with O₂ permeability of 9.5 mL O₂/m²/24 h at 0°C, 0.98 g/cm³ density was used in the current experiment. Five lots of sausage/bag and 8 packages for each treatment were used. The intended gases mixtures were purchased from a local gas supplier (Deokyang, Ulsan, Korea) in cylinders having an injection pipe and a gauge for the gas control system. One of the treatments was solely packed in a vacuum (Model 19/S, Röscherwerke Gmbh, Hanover, Germany) and used as a control treatment, and the other four treatments were sealed after flushing with the following gas mixtures: MAP1, 25% CO₂/75% N₂; MAP2, 50% CO₂/50% N₂; MAP3, 70% CO₂/30% N₂; MAP4, 100% CO₂. Residual gases were initially removed with vacuum and the sausages to gas volume ratio in the MAP samples were 1/1 (Gokoglu et al., 2010). Storage study was conducted for a total of 45 days and sampling was done at 1, 15, 30, and 45 days of storage time for physiochemical analyses [water activity, pH, color, volatile basic nitrogen (VBN) and thiobarbituric acid reactive substance (TBARS) contents, and texture profile analysis], microbial quality, and sensory characteristics of sausages samples. For each analysis time and batch, two packages of sausage were withdrawn, and each analysis was performed in triplicates.

After homogenizing three grams of a sample with 30 mL of distilled water in a homogenizer (Model Polytron® PT 2500 E Stand Dispersion Device, Kinematica AG, Malters, Switzerland), the pH values of the samples were determined. A digital pH meter (Mettler Toledo, Columbus, OH, USA) was used for reading the values. After slicing the core of the samples into 4 mm cubes, water activity (a_w) was assessed using a_w measurement apparatus (Lab master aw, Novasina AG, Lachen, Switzerland). Determination of VBN contents was performed according to the Conway micro diffusion method (Conway, 1950), and total VBN values were expressed in mg%. Analysis of the 2-TBARS, was conducted using the method indicated by Pikul et al. (1989), and the content was calculated as mg malonaldehyde equivalent per kg (mg MA/kg) of sample.

Instrumental color analysis was performed from the inner surface of the sliced sausages using a portable chromameter (CR-400, Konica Minolta, NJ, USA). Prior to the analysis, the device was calibrated using a standard calibration plate (Y=92.80, x=0.3136, and y=0.3194) and five readings per sample were taken for L* (lightness), a*(redness), b*(yellowness). The viewing/illuminating apertures were 11 mm/8 mm (8 mm) and 3 mm/3 mm, (3 mm) respectively. Average values were calculated from five readings and expressed as L*, a*, and b* based on the CIE color system (CIE, 1976).

Microbiological quality characteristics were conducted by enumeration of total plate count (TPC), lactic acid bacteria (LAB), Enterobacteriaceae, E. coli O157:H7, and Salmonella spp. Each dry fermented sausage sample was taken aseptically using a sterile spoon, combined with 225 mL of 0.1% peptone water, and homogenized for 2 min in a Stomacher Lab Blender (model 400 Circulator, Seward Laboratory Systems, Bohemia, NY, USA). Diluting 1 mL of the material in 9 mL of 0.1% sterile peptone water yielded a series of 10-fold dilutions (10¹ to 10⁷) After incubating samples with their appropriate selective medium, enumerations of the developed colony of microorganisms were undertaken. For TPCs, LAB, Enterobacteriaceae, E. coli O157:H7, and Salmonella spp. counts, the media used were Plate Count Agar (mbcell, Kisanbio, Seoul, Korea), Lactobacillus MRS agar (Difco, Becton, NJ, USA), Violet Red Bile Glucose Agar (VRBGA; Kisanbio), MacConkey Plates and Bismuth Sulfite Agar (Kisanbio) respectively and appropriate dilutions were incubated for 48 h in triplicate at 37°C (Drosinos et al., 2005). The average number of colonies per countable plate was determined, and the total number of CFU/g were calculated before the data was presented in Log CFU/g.

Color, lactic acid aroma, sourness, and overall acceptability of dry fermented sausages were all assessed using descriptive sensory analysis (scoring method). The sensory evaluation was performed by seven experienced panelists who are researchers and students in Daegu University’s Department of Animal Resources, Meat Science Laboratory. Ahead of the actual evaluation session, the panelists were trained on sensory characteristics of dry fermented sausages using five-point scale. The intensity scale used to define the quality attributes ranged from 1 to 5 that corresponds to the sensory attributes of samples as follows “extremely pale to very dark,” for color, “very weak fermented aroma to very strong fermented aroma,” for aroma, and “light sour to strong sour” for sourness. Three different types of commercial dry fermented sausage were used during training session, and panel were given 3 slices (5 mm thickness) of samples on white plastic plates during the judgment. To avoid carryover influences, all samples were individually labeled with three digits and provided at random. The sample consisted of five series. Each series was made up five batches manufacturing with the respective MAP gas mixture (0%, 25%, 50%, 70%, and 100% CO₂). Before each sample was examined, the panel were provided cold water to rinse their mouths. The sensory analysis method was certified by the life management committee of Daegu University and given an IRB number (1040621-201905-HR-004-02).

Statistical data were analyzed by using Analysis of Variance (ANOVA) for the three replicates. SAS software version 9.4 (SAS Institute, Cary, NC, USA) was used for the analysis, and a significance level of p<0.05 was applied for all evaluations. Differences among the means were compared according to Duncans’s multiple range test.

Effect of MAP on pH and a_w of dry fermented sausages during storage period is indicated in Table 1. MAP varying in gas composition had a significant (p<0.05) effect on the pH value of samples during storage. Similarly, Gokoglu et al. (2010) reported a significant difference in pH among the modified atmospheres during the storage. In all storage time, the batches in MAP samples presented significantly lower pH values than the vacuum-packed, control samples. The finding agrees with Kim et al. (2014) who observed a significantly lower pH in the MAP samples than that in the VP of dry-cured pork neck products at the given storage time. During the storage study, the pH value of MAP packages decreased as CO₂ concentration increased from 25% to 100% CO₂ in MAP1-MAP4 samples. The current result agrees with Cilla et al. (2006) and Martínez et al. (2005) reports that the increase in concentrations of CO₂ lowered pH in dry-cured meat products. The initial decrease of pH is due to CO₂ absorption. A possible reason, carbonic acid, H₂CO₃, may be produced from absorbed carbon dioxide by meat (Dixon and Kell, 1989), increased corresponding to the increase in CO₂ concentration in the MAP treatment and became responsible for the pH variation. Gokoglu et al. (2010) assumed that the increase in LAB count caused for decrease in pH values. In the current investigation, the LAB, which are responsible for lactic acid generation, showed a progressive decline in the MAP treatments as CO₂ concentration increased (Table 2), but the pH also fell, indicating that the pH value was unaffected by LAB. The storage time had a profound (p<0.05) effect on the pH value of all MAP treatments and vacuum-packed batches. The pH value notably decreased in all treatments as the storage time extended. This may be associated with lower LAB activity. Muhlisin et al. (2014) documented the increase in pH values of all groups MAP varying in gas composition as storage time increased after studying the effect of MAP on the shelf life of Longissimus dorsi. In the current study, the LAB was not completely inhibited in all treatments that lactic acid production at a steady rate and its accumulation as the storage time prolonged may be contributed for the decline in the pH. Houben and van-Dijk (2001) for sliced hams, Pexara et al. (2002) for cured turkey fillets, Kim et al. (2014) for dry-cured pork neck products, and Muhlisin et al. (2014) for Longissimus dorsi of Korean native black pigs during storage all testified the decrease of pH values in MAP products with extended storage time.

The chemical reactions and the survival of spoilage and pathogenic microorganisms rely on the a_w of the food products. Measurement of the a_w, therefore, increasingly important to determine the shelf-stability of meat products. a_w of treatments significantly varied on 1, 30, and 45 days of storage time. In the indicated days, all the MAP treatments regardless of their CO₂ composition exhibited the lower a_w as compared to vacuum-packed treatment. The current finding agrees with Kim et al. (2014) that the a_w values of the MAP samples were significantly lower than those of the VP samples after 30, 60, and 90 days of storage. At day 1 of storage, MAP4 had the lowest a_w than other MAP treatments. When the storage time prolonged to 30 and 45 days, MAP3 and MAP4 presented similarly lowest a_w than all the treatment. The decrease in a_w in MAP treatments was corresponding with the increase in CO₂. After application of MAP to reduce the ripening time of dry-cured boneless hams, Wang (2001) indicated that the stability of microbiological quality is attributed to low water activity in dry-cured ham and bacteriostatic effect of modified atmospheres. Storage time had a significant effect on the a_w of all MAP treatments regardless of their gas composition and the values noticeably decreased as the storage time prolonged. In the vacuum-packed samples, a_w increased at day 30 storage compared to earlier storage of days 1 and 15. However, it decreased again at 45 days of storage. Similarly, Rubio et al. (2006) observed a decrease in a_w values of sliced dry-cured meat under both VP and MAP conditions when the storage time was extended.

Effect of MAP varying in CO₂ and N₂ composition compared to the control, VP, on microbial quality of dry fermented sausages during storage (45 days) indicated in Table 2. All MAP batches showed a lower TPC than vacuum-packed treatments throughout the storage period and the effect was significant (p<0.05) at day 1 and day 30 of the storage time. Microbial inhibition was effective as CO₂ composition grew from 25% to 100% in MAP1-MAP4 treatments throughout these days, and the effect was significantly (p<0.05) higher in MAP3 and MAP4 than other MAP treatments and the vacuum packaged control. Similarly, Gokoglu et al. (2010) reported the lowest count in samples packed under 100% CO₂ after studying the effect of MAP on the quality and shelf life of frankfurter type-sausages. Kim et al. (2014) observed significantly lower total aerobic bacteria and LAB counts in MAP samples than those of the VP samples. It has been stated by Sørheim et al. (2004) that a concentration of 20%–30% CO₂ was sufficient to prevent the growth of aerobic spoilage bacteria. In the current study, the effective inhibition could be achieved starting with the use of 25%, that is MAP1, and confirmed to the earlier finding; however, the effect gets pronounced as the concentration of CO₂ increased. The progressive reduction in a_w along with the increase in CO₂ of MAP treatments could be the possible reason for the decline in microbiological counts presented in the present study (Table 2). Storage time has a profound effect on the TPC of all MAP treatments and the control. TPC substantially increased (p<0.05) as the duration time extended in all treatment batches.

LAB count showed a similar trend to TPC. There is a noticeable (p<0.05) difference in LAB counts of MAP treatments varying in their gas mixture across the storage period (Table 2). In all the storage days, vacuum-packed samples had a significantly highest LAB count than all MAP packages regardless of their gas compositions. Similarly, Kim et al. (2014) documented that the LAB counts in MAP samples were significantly lower than those of the VP samples in dry-cured pork neck products. According to Aksu et al. (2005), MAP with CO₂ and N₂ greatly inhibited the growth of LAB. The prominent inhibitory effect for LAB starts in MAP1 packages, and the effect increased as the increment of CO_2, and a significant highest count is exhibited in MAP4 with 100% CO_2. It was recognized that the rate of carbon dioxide in the gas mixture affected the growth of LAB (Gokoglu et al., 2010). Previous investigations of Blickstad and Molin (1984), Borch et al. (1996), and Metaxopoulos et al. (2002) have reached similar conclusions. In contrast, some researchers exhibited that MAP did not have a growth rate hindering effect on the LAB compared to VP (Pexara et al., 2002; Samelis and Georgiadou, 2000). Gokoglu et al. (2010) reported that a higher aerobic spoilage bacteria inhibition effect was observed when the gas mixture contained over 30% CO₂. The current finding, inhibition of LAB was achieved starting from 25% CO₂ composition which is comparable with the previous study. Storage time had a noticeable (p<0.05) effect on LAB counts of all MAP treatments and the control, VP. In all the batches, the count substantially decreased as storage time extended. However, dry fermented sausage is manufactured with deliberate addition of the LAB to achieve the important characteristics required for dry fermented sausages, further activity of the LAB is not required after products completed the ripening process to maintain the quality. In this regard, the decrease in LAB counts by the application of MAP and the storage time can be appreciated in keeping the quality of the products.

Evaluation of the microbiological quality and safety of food products are commonly carried out by determination of indicator microorganisms’ levels and the one is Enterobacteriaceae (EFSA, 2010; Moore and Griffith, 2002). The Enterobacteriaceae are a large family of facultative anaerobic, Gram-negative bacilli that inhabit the intestines of many animal species. This family includes pathogenic Escherichia, Salmonella serovars, and Klebsiella species (Gwida et al., 2014; Ruby and Ingham, 2009). The high prevalence of Enterobacteriaceae could be attributed to inadequate sanitary conditions and poor general hygiene. MAP treatments varying in their gas mixture showed a significant (p<0.05) effect on Enterobacteriaceae counts at 1, 15, and 45 days of storage days (Table 2). On day 1, VP, MAP1, MAP2 had similar higher counts than MAP3 and MAP4 batches. In prolonged storage of 15 and 45 days, however, all MAP packaging resulted in a significantly lower Enterobacteriaceae count than the vacuum-packed samples and counts in MAP3 and MAP4 samples were markedly (p<0.05) lower than all other treatments during the stated period. According to Kim et al. (2014), MAP with a combination of CO₂ and N₂ inhibited the growth of LAB and Enterobacteriaceae. All MAP packaging presented a decrease in the counts at the final storage time of 45 days compared to day 1 storage time when the count increased for VP samples.

Counts for E. coli O157:H7 and Salmonella spp. was exhibited during day 1 in all MAP treatments and vacuum-packed batch. E. coli O157:H7 count ranged from 1.43 Log CFU/g in MAP4 to 2.71 Log CFU/g in vacuum-packed samples, and the range for Salmonella spp. count was 2.55 Log CFU/g in MAP3 to 2.87 Log CFU/g in vacuum-packed batch. Gram-negative bacteria are generally more sensitive to CO₂ than Gram-positive bacteria (Church, 1994) because most Gram-positive bacteria are facultative or strict anaerobes (Gill and Tan, 1980). Despite, the variation was not significant, vacuum-packed samples resulted in higher E. coli O157:H7 and Salmonella spp. counts than all MAP treatments at early 1-day storage time. Vacuum-packed samples were exhibited to have higher Enterobacteriaceae (Table 2) which includes E. coli O157:H7 and Salmonella spp. at day one of the storage. As the storage time extended to 15, 30, and 45 days, both E. coli O157:H7 and Salmonella spp. disappeared in all MAP and vacuum-packed treatments.

Table 3 displays the effect of MAP varying in CO₂ and N₂ composition compared to the control, VP, on TBARS and VBN contents of dry fermented sausages during the storage period (45 days). Martínez et al. (2006) and Gokoglu et al. (2010) documented those sausages are more sensitive to oxidation than intact muscle because grinding reduces particle size and disrupts membranes, allowing air and oxygen to enter the tissues. Treatments had a significant (p<0.05) variation in TBARS content across the storage period. Vacuumed-packed samples presented the highest TBARS content on 1, 15, and 30 days of storage time and along with MAP2, 50% CO₂ and 50% N₂, at the final storage time as compared to other treatments. The current finding is in agreement with Wang et al. (1995) who found less oxidation in modified-atmosphere packed samples than those in vacuum packed. In contrast, higher TBARS values under MAP than those packed under vacuum was reported by some researchers (Berruga et al., 2005; Gokoglu et al., 2010; Kerry et al., 2000; Martíinez et al., 2006). On day 1 of storage, MAP4 showed a significantly (p<0.05) lowest TBARS value than all treatments and other MAP treatments were the same in the content. The increase in concentration of CO₂ affected lipid oxidation. Based on the TBARS content at day 15 and 30 storage time, treatments are ranked as follows: Vacuum-packed>MAP1>MAP2>MAP3>MAP4 as the prevention of rancidity through an increase in CO₂ concentration. Jeremiah (2001) documented that the occurrence of lipid oxidation can be prevented by anaerobic packaging that the present study could achieve the inhibition of rancidity by increasing the CO₂ concentration. During the storage period of 45-days, except for MAP2 treatment which had the same highest content as vacuum-packed, MAP treatments showed a decrease in TBARS content as the increase in CO₂ composition and MAP4 exhibited the lowest content throughout the storage study. Correspondingly, Gokoglu et al. (2010) reported the oxidation inhibition effect of carbon dioxide concentration based on TBARS analysis. The TBARS values significantly (p<0.05) decreased in vacuum-packed, MAP3 and MAP4 samples, and increased in MAP3 treatment while the MAP1 batch was unaffected (p>0.05) due to extended storage time. The increased TBARS value in MAP2 samples indicates rancidity development when rancidity was inhibited in vacuum-packed, MAP3 and MAP4 samples during the storage.

The VBN content indicates protein degradation and the increase of VBN content in meat can be caused by either bacterial or enzymatic degradation of proteins (Egan et al., 1981; Kim et al., 2014). In the current study, the VBN values significantly (p<0.05) varied among treatments up to 30 days of storage time and the variation disappeared and all the treatments at the final storage time (Table 3). Across the study period, the variation didn’t show similar trend and the values of treatments were fluctuating inconsistently having no relation with the composition of gas used in MAP and the bacterial growth characteristics (Table 2) during storage. However, the MAP2 treatment with 50% CO₂ and 50% N₂ gas mixture had the highest VBN content throughout storage period. The highest VBN and TABRS content exhibited in MAP2 could be the reason for the lowest sensory attributes of color and overall acceptability of the samples as to the panel judgment in the current study. Additionally, the change in storage time didn’t show a clear trend in the VBN contents of the vacuum-packed samples and all the MAP treatments regardless of their gas composition. The VBN value noticeably (p<0.05) increased from day 1 up to 30 days and then decreased at the final storage time for MAP1, MAP3, and MAP4 treatments. The VBN content for MAP2 increased on 15 and 30 days compared to initial storage time thereafter decreased significantly (p<0.05) in the final storage time like other MAP treatments. The VBN content of the vacuum-packed samples increased at 30 days compared to the previous time but then declined substantially (p<0.05) at the final storage time. All treatments tended to have a decrease in VBN at the final storage time (45 days).

Color is an important qualitative factor that determines meat and meat products acceptability of consumers (Glitsch, 2000; Gokoglu et al., 2010). The three primary (L*, a*, and b*) color coordinates used in the Hunter system of color determination were performed in the current study and the results are presented in Table 4. A significant variation in the L* color attribute of treatments was observed during days 1 and 15 of the storage time and the variation disappeared thereafter at 30 and 45 days of storage time. On days 1 and 15 storage time, all MAP treatments showed a noticeable (p<0.05) lower score in L* than the vacuum-packed samples. Similarly, Kim et al. (2014) observed the highest L* in VP samples than the MAP samples at all storage times except at Day 45. Gokoglu et al. (2010) found lower L* in samples packed under 30% CO₂/70% N₂ and 100% CO₂ atmospheres during the storage. In contrast, Rubio et al. (2007) and García-Esteban et al. (2004) documented that the type of packaging system had little influence on L*, and a significant difference was not found between vacuum-packaged and MAP treatments. Our results disagree also with Li et al. (2012) who reported higher L* of beef MAP than VP. As a result of metmyoglobin production, elevated CO₂ concentrations in MAP cause a degree of discoloration. The redox chemistry of myoglobin can be altered by gases in MAP, which affects color. In the current study, the variation among the MAP samples was not substantial regardless of the difference in gas composition applied. The L* of samples packed with MAP tended to decrease as the storage time extended and the values were significantly (p<0.05) higher at day 1 storage than further storage period for all MAP samples varying in gas composition. The vacuum-packed samples significantly (p<0.05) decreased in L* on 15 and 30 days of storage than at day 1. However, the value again increases at 45 days but still maintained a lower value as compared to day one. The current finding disagrees with García-Esteban et al. (2004) who reported the increased L* in vacuum packed samples and the stability in the modified atmosphere packed samples during storage.

In meat and meat products, redness (a*) is considered a color stability indicator (Kim et al., 2014). There was a significant difference in a* among MAP, varying in CO₂ and N₂ composition, and the vacuum-packed batches on 1, 30, and 45 storage days, and vacuum-packed samples exhibited a decrease in a* than all MAPs treatments. Hur et al. (2013) stated that CO₂ has a positive role in the reduction of lipid oxidation and negative effects in color deterioration in meat packaging during storage. During the storage time, a* of MAP4 was higher than all other treatments, and other MAP packages presented similar values which were higher than the vacuum-packed samples. The current finding is in agreement with El Adab et al. (2020) who observed an increase in a* during the storage of sausages packaged under modified atmospheres. Similarly, Jeremiah et al. (1995) reported that pork packaged with 100% CO₂ had great color stability. On the contrary, Ruiz-Capillas and Jiménez-Colmenero (2010) reported that a* remain constant during the storage of meat products packaged under modified atmosphere. Viana et al. (2005) reported that high CO₂ concentrations in meat MAP application as the major disadvantage with a certain degree of darkening as a result of metmyoglobin formation. Sørheim et al. (1997) reported that CO₂ did not effect on meat color. According to Hur et al. (2013), a decreased redness is associated with rancidity. The decreased TBARS values in the MAP samples of the current study (Table 3) can be related to the increase in a* which indicates the advantage of MAPs in color stability by inhibiting the oxidation of lipids. Storage time had a profound (p<0.05) effect in a* of all the packages, MAP, and vacuum-package, used in the current study. And a marked decline in redness was observed in all samples at 30 and 45 days than earlier storage time. The present study is in agreement with Kim et al. (2014) who reported a pronounced fading in the redness color of all packaging systems, both VP and MAP samples, at extended storage time. In contrast, Esturk and Ayhan (2009) reported a decrease in the redness of salami slices at all MAP applications with an increase in storage time.

The yellowness (b*) value of treatments significantly showed variation during day 1, 15, and 30 storage time and all MAPs samples exhibited lower b* than vacuum-packed batch. All MAP samples regardless of the gas composition had similar value during the stated period. Then after, all the MAP and vacuum-packed samples presented a similar b* on the final storage time of 45 days. The current finding disagrees with Kim et al. (2014) report who found a significantly higher b* in the MAP samples than the VP samples at day 30, 60, and 90 of storage. Cilla et al. (2006) reported the increase in yellowness color in MAP samples is related to the increased pigment oxidation during storage. Martínez et al. (2005) has been demonstrated that myoglobin oxidation is favored as the concentrations of CO₂ increased. However, the TBARS analysis of the current study (Table 3) didn’t support the stated hypothesis as MAP treatments showed a decrease in TBARS content as the increase in CO₂ composition having the lowest content in MAP4, 100% CO₂, throughout the storage study. Similarly, Wang et al. (1995) reported that TBA and peroxide values were lower in modified atmosphere than in vacuum conditions after analyzing the lipid oxidation in Chinese-style sausages both stored at 4°C and 15°C temperatures. All the MAP and vacuum-packed samples showed a decrease in b* due to extended storage time and the values at day 45 storage was substantially lower than day 1 storage time. Similarly, Gokoglu et al. (2010) reported a decreased b* of the samples packed under modified atmosphere and vacuum during storage.

Effect of MAP varying in CO₂ and N₂ composition compared to VP on sensory characteristics of color, lactic acid aroma, sourness, and overall acceptability attributes of dry fermented sausages during storage was investigated, and the results are presented in Table 5. Treatments showed a significant (p<0.05) variation in color attribute of sensory characteristics on 1, 30, and 45 storage days, lactic acid aroma and sourness on 1^st day of storage, and overall acceptability at the initial and final storage period. The current results disagree with those of Fernández-Fernández et al. (2002) who documented that packing methods did not affect any sensory property of dry sausages subjected to VP and MAP. The color was preferred in vacuum packed and MAP1 on day 1 and MAP3 joined the preferred group on day 30 and 45 days of storage. The color attribute in MAP2, 50% CO₂ and 50% N₂, samples were lower compared to all other treatments. The sensory color score significantly decreased in all MAPs due to extended storage and vacuum-packed samples did not vary across the storage time. On day 1, the lactic acid aroma in vacuum packed, MAP1 and MAP2 were noticeably (p<0.05) higher than others, and sourness were preferred in all MAPs than vacuum-packed samples. Thereafter, variation was not detected (p>0.05) among treatments in both sensory traits of the lactic acid aroma and sourness according to the panel’s judgments. Both sensory traits of lactic acid aroma and sourness values were significantly (p<0.05) decreased in all treatment samples, MAP sample and vacuum-package, due to prolonged storage, and the lowest scores were recorded in the final storage time of 45 days. The overall acceptability score in MAP2 samples was the lowest compared to all other treatments in the initial and final storage days and no variation was exhibited among other treatments during these days. The least score exhibited in color and overall acceptability of MAP2 samples can be related to the higher TBARS and VBN results (Table 3) which is associated with lipid oxidation and protein degradations in the treatment. The overall acceptability of MAP1, MAP3, and MAP4 samples did not show changes due to storage time, samples in vacuum-packed and MAP2 samples decreased significantly at final storage time than the initial day 1 storage.

In conclusion, the use of MAPs showed a better microbial inhibition than vacuum package with an increase in the CO₂ from 25% to 100% in MAP1-MAP4 samples. Modified atmospheric packaging with 70% CO₂/30% N₂ (MAP3) and 100% CO₂ (MAP4) are found to be more effective to maintain several quality parameters (a_w, pH, microbial inhibition, stability against lipid oxidation, and instrumental color traits) of dry fermented sausage and extend the shelf life without any effect on sensory quality characteristics during storage.