Article

The L-ascorbic acid increases proliferation and differentiation of Yanbian cattle skeletal muscle satellite cells by activating the Akt/mTOR/P70S6K signaling pathway

Huaina Jin1,, Qiang Li1,, Lin Tang1,, sajida Naseem1, Sungkwon Park2, Enze Wang1, Bin Sun1, Abid manzoor1, Sun Jin Hur3, Xiangzi Li1,*, Seong-Ho Choi4,*
Author Information & Copyright
1Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Department of Animal Science, Yanbian University, Yanji 133002, China.
2Department of Food Science and Biotechnology, Sejong University, Seoul 05006, Korea.
3Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
4Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea.

† These authors contributed equally to this work.

*Corresponding Author: Xiangzi Li. E-mail: lxz@ybu.edu.cn.
*Corresponding Author: Seong-Ho Choi. E-mail: seongho@cbnu.ac.kr.

© Copyright 2024 Korean Society for Food Science of Animal Resources. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: May 16, 2024 ; Revised: Jun 16, 2024 ; Accepted: Jun 18, 2024

Published Online: Jul 17, 2024

Abstract

Cell-cultured meat, as a new type of meat food, can effectively mitigate the negative effects of conventional animal husbandry on the environment, health and animal welfare. Muscle stem cells are the main seed cells for the production of cell-cultured meat, but their weak proliferative capacity in vitro severely limits the large-scale and low-cost production of cell-cultured meat. There is growing evidence that L-ascorbic acid (AA) has the ability to increase the efficiency of muscle stem cell proliferation and differentiation in vitro. However, the role of AA in Yanbian bovine skeletal muscle satellite cells (BSCs) and its molecular mechanisms are unknown. Therefore, in the present study, the promotional effect of AA on the proliferation and differentiation of BSCs was confirmed by the Cell Counting Kit 8 (CCK-8) assay, 5-ethyl-2′-deoxyuridine (EdU) proliferation assay, real-time fluorescence quantitative PCR (RT-qPCR), immunoprotein blotting (Western blotting) and immunofluorescence assay. RT-qPCR and Western blotting results showed that AA up-regulated the expression of phosphorylated (p)-Akt, p-mTOR, and p-P70S6K genes and proteins, whereas when the mTOR pathway inhibitor rapamycin was co-treated with AA in BSCs, the expression of p-Akt, p-mTOR, and p-P70S6K genes and proteins was significantly down-regulated. In summary, data suggest that AA regulates the proliferation and differentiation of BSCs by activating the AKT/mTOR/P70S6K signaling pathway. These data provide a practical approach and theoretical basis for the efficient and low-cost manufacture of cell culture meat.

Keywords: cell proliferation; cell differentiation; L-ascorbic acid; rapamycin pathway; skeletal muscle satellite cells